sup-b15細胞，ATCC人Ph+急淋白血病細胞

產(chǎn)品型號：
產(chǎn)品時間：2024-08-09
簡要描述：慧穎生物供應(yīng)美國ATCCsup-b15細胞人Ph+急淋白血病細胞（ATCC CRL-1929），C3-C5代數(shù)，懸浮樣，器官來源：骨髓，淋巴樣，狀態(tài)良好，*，提供*技術(shù)指導(dǎo)，提供免費復(fù)蘇服務(wù)，售后齊全，敬請放心購買！

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sup-b15細胞，ATCC人Ph+急淋白血病細胞 簡介：

產(chǎn)品名稱：sup-b15細胞

中文名稱：人Ph+急淋白血病細胞

來源：美國ATCC

貨號：ATCC CRL-1929

類別：細胞系，傳代細胞

種屬：人

生長狀態(tài)：懸浮生長

年限：8 years

細胞形態(tài)：淋巴樣

數(shù)量：大量

細胞類型：B淋巴細胞

是否是腫瘤細胞：0

相關(guān)疾病:白血病

運輸方式：凍存運輸

器官來源：骨髓

代數(shù)：C3-C5

sup-b15細胞，ATCC人Ph+急淋白血病細胞 英文描述：

Designations:	SUP-B15
Depositors:	SD Smith
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	suspension
Organism:	Homo sapiens
Morphology:	lymphoblast
Source:	Organ: bone marrow Disease: acute lymphoblastic leukemia Cell Type: B lymphoblast;
Cellular Products:	immunoglobulin (cytoplasmic)
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression:	CD1a -; CD2 -; CD3 -; CD4 -; CD5 -; CD8 -; CD10 +; CD13 +; CD38 +; CD71 +; HLA DR +
DNA Profile (STR):	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,14 D16S539: 11,12 D5S818: 12,13 D7S820: 10,11 THO1: 6,9.3 TPOX: 8,9 vWA: 15,17
Cytogenetic Analysis:	46, XY; the following markers are present: t(9;22)(q34;q11), t(4;14) (p11;q24), der(4)t(1;4) (p11;q33), t(9;22) (q34;q11), der(10)t(3;10) (q25;q26), add(16); Philadelphia chromosome is present.
Age:	8 years
Gender:	male
Ethnicity:	Caucasian
Comments:	This line was was derived from malignant cells collected from the bone marrow of an 8 year old child with Philadelphia chromosome positive B cell ALL. The cells express multiple B lineage markers, but do not express T cell markers. The cells are positive for the beta-2-microglobulin, Leu12, My7 (CD13), OKT9 (CD71), OKT10 (CD38) and CALLA (CD10) antigens. [23068 ] They are are negative for CB1, Leu 1 (CD5), Leu2 (CD8), Leu3 (CD4), Leu4 (CD3), Leu5 (CD2), Leu6 (CD1a), Leu9, Leu M1 (CD15), My9 (CD33), surface immunoglobulin (sIg -) and Epstein-Barr virus. [23068 ]
Propagation:	ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20% Temperature: 37.0°C
Subculturing:	Protocol: Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 5 X 10 exp5 viable cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml. Medium Renewal: 2 to 3 times per week
Preservation:	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature
Doubling Time:	18 hrs
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020
References:	22924: Fainstein E, et al. A new fused transcript in Philadelphia chromosome positive acute lymphocytic leukaemia. Nature 330: 386-389, 1987. PubMed: 2825022 22958: Clark SS, et al. Expression of a distinctive BCR-ABL oncogene in Ph1-positive acute lymphocytic leukemia (ALL). Science 239: 775-777, 1988. PubMed: 3422516 23068: Naumovski L, et al. Philadelphia chromosome-positive acute lymphoblastic leukemia cell lines without classical breakpoint cluster region rearrangement. Cancer Res. 48: 2876-2879, 1988. PubMed: 3162827 23302: Rubin CM, et al. Heterogeneity of genomic fusion of BCR and ABL in Philadelphia chromosome-positive acute lymphoblastic leukemia. Proc. Natl. Acad. Sci. USA 85: 2795-2799, 1988. PubMed: 2833755 23305: Kawasaki ES, et al. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc. Natl. Acad. Sci. USA 85: 5698-5702, 1988. PubMed: 3165197